Department of Human Genetics

Research interest of the Department mainly concerns:

Studies on biological mutagenesis and the role of reparative systems in the renovation of genetic damages in cells of both pro-and eukaryotic origin.

Ongoing research projects are focused on:

• Studies on regulatory mechanisms of cytotoxic and mutagenic activity of alkylating compounds with the aim of optimisation of the chemotherapy of oncological diseases.
• Elaboration of technology for isolation, cultivation and differentiation of human stem cells for further application in gene and cell therapy, including.
• New approaches for enhancing the resistance of biosystems to environmental and professional hazards are being intensively developed.
• Design of mutation process models and experimental samples of computer programs for data base processing.

Selected publications:

1. Lukash L.L., Varshaver N.B., Buzhievskaya T.I., Shapiro N.I. The oncogene of BAV-3 as mutagen // J. Cell Science. – 1985. – 78. – P.97 – 103.
2. Lukash L.L., Buzhievskaya T.I. Role of early viral genes in mutagenesis // Biotechnology Current Progress. – Lancaster-Basel.: Technomic Publishing Co., Inc., 1991. – P.119 – 131.
3. Lukash L.L., Boldt J., Pegg A.E., Dolan M.E., Maher V.M., McCormick J.J. Effect of O6-alkylguanine-DNA-alkyltransferase on the frequency and spectrum of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the HPRT gene of diploid human fibroblasts // Mutation Research. – 1991. – P.397 – 409.
4. Kordyum V.A., Frolkis V.V., Lukash L.L., Shulzhenko V.N., Shpilevaya S.P., Kostetsky I.E., Titok T.G., Varsanova I.S., Likhacheva L.I., Irodov D.M. Gene therapy of mass patologies // Biopolymers and cell. – 1993. – 9, №4. – P.63 – 104.
5. Lukash LL, Karpova IS, Miroshnichenko OS, Tikhonova TN, Lylo VV, Man'ko VG, Sukhorada EM, Golynskaia EL. The effect of the lectin from Sambucus nigra inflorescences on spontaneous and alkylating agent-induced mutagenesis in mammalian somatic cells // Cytology and Genetics. – 1997. – 31, №5. – P.52 – 60.
6. Karpova IS, Pidpala OV, Lukash LL. The DNA of recombinant plasmids acquires mutagenic activity in a Bacillus subtilis culture after the insertion of the human genome Alu repeat // Cytology and genetics. – 1999. – 33, №4. – P.3 – 8.
7. Lukash L.L., Vasilovskaya S.V. Mammalian stem cells in vitro as a basis for the development of a new biotechnologies // Biopolymers and cell. – 2001. – 17, №3. – P.203 – 211.
8. Lukash L.L., Lylo V.V., Man'ko V.G., Terentev A.G. The increasing mutagenic effect of nitrosoguanidine under influence of modified bases during inhibition of repair AGT enzyme in mammalian somatic cells in vitro // Cytology and Genetics. – 2002. – 36, №3. – P.41 – 50.
9. Chernenko V.Yu., Lukash L.L., Macewicz L.L., Kochubey T.P., Elesichev A.A., Klimenko E.A., Gorban L.N. Application of DNA microelectrophoresis for estimation of genotoxic effects of mutagens in the culture of human haemopoietic cells // Cytology and Genetics. – 2004. – 38, №1. – P.31 – 35.
10. Lukash L.L. The regulation of variability of somatic mammalian cell genome under the influence of exogenous biological factors // Biopolymers and cell. – 2004. – 20, №1-2. – P.93 – 105.