Laboratory of Biomolecular Electronics

We are pursuing investigation in the areas:

Scientific and technological foundations for the creation of novel analytical systems (bio- and chemosensors) based on the electrochemical or optical transducers and biological material as well as the development of artificial biomimics as sensitive elements.

Our current research activities:

• Study on molecular basis of the interaction of enzymes with substrates and inhibitors using electrochemical sensors. The interplay of immobilized enzymes with substrates and inhibitors has been investigated and the core kinetic characteristics of enzymes have been determined.
• Fabrication of a test-system on the basis of electrochemical biosensors used for the determination of trace concentration of common toxins. Potentiometric and conductometric enzymatic biosensors have been designed and their laboratory prototypes have been generated aimed at direct and inhibitor analysis of herbicides, pesticides, steroid glycoalkaloids, formaldehyde, heavy metal ions and other substances in food and environment.
• Design of novel microbiosensors for the determination of main metabolites and the monitoring of some neurotransmitters. Amperemetric biosensors have been created for the detection of i) glucose, ethyl alcohol and lactic acid in vitro (in the Crimean and other wines), ii) acetylcholine, choline and glutamine acid in the brains of experimental animals.
• Generation and exploration of the synthetic analogues of biological receptors using the method of molecular imprinting and their application in sensor technology, solid-phase extraction and chromatography. The methods of polymers-biomimics synthesis in the form of thing films and membranes have been worked out. These polymers-biomimics were used as basis for fabricating electrochemical, (conductometric, capacitive and amperometric) sensors for further detection of herbicides belonging to the triazine family.
• Development of electrochemical and optical immuno- and DNA-sensors. Conductometric and potentiometric sensors for IgG and recombinant α2-interferon determination have been designed. Our immunosensor devices have been successfully tested on blood serum analysis and cultural media.

Selected publications:

1. S.V.Dzyadevych, N.Jaffrezic-Renault. Conductometric Microbiosensors for Environmental Monitoring. Sensors.- 2008.- V.8.-P. 2569-2588.
2. V.N.Arkhypova, S.V.Dzyadevych, N.Jaffrezic-Renault, C.Martelet, A.P.Soldatkin. Biosensors for assay of glycoalkaloids in potato tubers. Applied Biochemistry and Microbiology.- 2008.- V.44, No 3.- P. 314-318.
3. S.V.Dzyadevych, A.P.Soldatkin. Solid-State electrochemical enzyme biosensors. Kyiv: IMBG.- 2008.- 222 p
4. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Conductometric enzyme biosensors. Handbook of Biosensors and Biochips / Eds R..S.Marks, C.R.Lowe, D.C.Culen, H.H.Weetall, I.Karube.- Wiley-Interscience.- 2007.- P. 379-394.
5. S.V.Dzyadevych, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Enzyme biosensors based on ion-selective field-effect transistors. Analytica Chimica Acta.- 2006, 568.- P.248-258.
6. A.P.Soldatkin, S.V.Dzyadevych, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Pathways for improving potentiometric and conductometric enzymatic biosensors. In: Encyclopedia of Sensors (Eds. C.A.Grimes, E.C.Dickey, M.V.Pishko).- California, USA: American Scientific Publishers.- 2006.- V.7.- P. 331-348
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7. С.В.Дзядевич, О.П.Солдаткін. Наукові та технологічні засади створення мініатюрних електрохімічних біосенсорів. Київ: Наукова думка.- 2006.- 261 с.
8. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, A.V.El’skaya. Electrochemical enzyme biosensors. Kyiv: IMBG.- 2006.- 72 p.
9. L.V.Shkotova, A.P.Soldatkin, M.V.Gonchar, W.Schuhmann, S.V.Dzyadevych. Amperometric biosensor for ethanol detection based on alcohol oxidase immobilized within electrochemically deposited resydrol film. Material Science and Engineering C.- 2006.- 26.- P.411-414.
10. A.P.Soldatkin, V.N.Arkhypova, S.V.Dzyadevych, A.V.El’skaya, J.-M.Gravoueille, N.Jaffrezic-Renault, C.Martelet. Analysis of the potato glycoalkaloids by using of enzyme biosensor based on pH-ISFETs. Talanta.- 2005, 66, P. 28-33.
11. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, A.V.El’skaya, J.-M. Chovelon, C.Georgiou, C.Martelet, N.Jaffrezic-Renault. Early-warning electrochemical biosensor system for the environmental monitoring based on enzyme inhibition effect. Sensors and Actuators B, 2005, 105, P. 81-87.
12. O.N.Schuvailo, S.V.Dzyadevych, A.V.El’skaya, S.Gautier-Sauvigne, E.Csoregi, R.Cespuglio, A.P.Soldatkin. Carbon-fibre-based microbiosensors for in vivo measurements of acetylcholine and choline. Biosensors & Bioelectronics.- 2005.- 21, P. 87-94.
13. S.V.Dzyadevych, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, J.-M.Chovelon, A.V.El’skaya, A.P.Soldatkin. Potentiometric biosensors based on ISFETs and immobilized cholinesterases. Electroanalysis, 2004, 16, P. 1873-1882.
14. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Development and optimisation of biosensors based on pH-sensitive field effect transistor and cholinesterase for sensitive detection of solanaceous glycoalkaloids. Biosensors & Bioelectronics.- 2003.- 18.- P. 1047-1053.
15. S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, V.N.Arkhypova, A.V.El’skaya, J.-M.Chovelon, C.Martelet, N.Jaffrezic-Renault. Biosensors based on enzyme field effect transistors for determination of some substrates and inhibitors. Analytical and Bioanalytical Chemistry.- 2003.- 377.- P. 496-506.