Laboratory of Molecular Mechanisms of Autoimmunity

Head

Lyudmila L. Sidorik

Ph.D. (Mol. Biol.), Senior Staff Scientist
Phone: (380-44) 526-55-89;
Fax: (380-44) 526-07-59;
E-mail: sidorik@imbg.org.ua

Education and Degrees:

1974–1979 Graduate Student, Faculty of Biology, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, M.Sc. (biophysics)

1990 Ph.D. (molecular biology)

Professional Employment:

1979–1984 Engineer, Department of Structure and Functions of Nucleic Acids (DSFNA), Institute of Molecular Biology and Genetics (IMBG), NASU, Kyiv, Ukraine

1985–1989 Junior Research Scientist, DSFNA, IMBG NASU, Kyiv, Ukraine

1990 Senior Research Scientist, DSFNA, IMBG NASU, Kyiv, Ukraine

1991 Head of Immunochemistry Group, DSFNA, IMBG NASU, Kyiv, Ukraine

1992 Fellowship of INSERM (Institut National de la Santé et de la Recherche Médicale), Pasteur Institute, Paris, France (3 months)

1993 Fellowship of INSERM, Pasteur Institute, Paris, France (2 months)

since 1995 Head of Lecture course in Molecular and Cellular Immunology, Faculty of Biology, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

since 2002 Head of the Laboratory of Molecular Mechanisms of Autoimmune Processes, IMBG NASU, Kyiv, Ukraine

Research Area:

Molecular chaperons as universal regulators of eukaryotic stress-induced signaling

Сurrent Research Activities and Recent Achievements:

  • The development of experimental animal models of inducible heart failure (various stages of inducible myocarditis and inducible DCM-like myocardial injury) – for primary screening of bioactivity, possible cardiotoxicity and/or immunogenicity of new pharmaceutic drugs and nanotechnologies.
Fig. 1. Probable binding configuration of Hsp60 with Akt1
  • Study on peculiarities of molecular chaperons Hsp60 and Hsp90 expression, function and localyzation and their regulatory role in apoptotic signaling of cardiomyocytes at heart failure progression.
Fig. 2. Anti-Hsp60 and anti-Hsp90 autoantibodies level determined by ELISA method in sera of patients affected by DCM, ischemic disease (IHD) and healthy donors (HD)
  • Study on chaperonin Hsp60 proinflammation biological activity of patients with congestive heart failure and women with chronic infertility.
  • Creation of bank of recombinant chaperons and specific anti-chaperons antibodies for development of diagnostic tools for verification of heart failure different stages, prognosis of severe arterial hypertension progression.
Fig. 3. Hsp90 localization in left ventricular of DCM-affected (A) and normal (B) human myocardia detected by immunohystochemical method. Magnification x 100

National Grants:

Projects of National Academy of Sciences of Ukraine:

  • 2012–2016 N 2.2.4.20 Project: “Structure-functional peculiarities of Hsp90 interaction with targets in heart failure pathogenesis” (scientific supervisor – Sidorik L. L.)
  • 2010–2014 N 102.10-Н Project: “Evaluation of biocompatibility and biosecurity of metal nanoparticles in vitro and in vivo, perspective as cargo targeting of cardiogeneic and antiapoptotic drugs” (scientific supervisors – Director of F. D. Ovcharenko Institute of Biocolloidal Chemistry, NASU, (Kyiv); Dr.Sci. (chem.), Prof. Ulberg Z. R.)
  • 2007–2011 N 2.2.4.20 Project: “Study of peculiarities of molecular chaperon Hsp90 expression and function using experimental model of chronic stress”
  • 2003–2006 N 2.2.4.20 Project: “Investigation of cytoplasmic and mitochondrial chaperons’ function at dilated cardiomyopathy”

Projects of State Fund for Fundamental Researches:

  • 1997–1998 N 1.1.2/2225 Project: “The study of autoantibodies directed against protein biosynthesis components at systemic autoimmune diseases”
  • 1993–1994 N 5/357 Project: “Immunochemical investigation of structure and function peculiarities of cytoplasmic rybonucleoproteines”
  • 1991–1993 Project: “The study of heart and vessels injury’ molecular and cellular mechanisms at cardio-vascular pathologies”

Projects of Ministry of Education and Science of Ukraine:

  • 2010–2011 Ukraine-Poland scientific and technical cooperation N M/135-2009 Project: “Autoantibodies against Hsp60, Hsp90 and Sgt1 as new diagnostic tool in dilated cardiomyopathy”

International Grants:

  • 2013–2017 GDRI (International Research Networks) N UMR 8203 Project: “New pharmacological approach by siRNA-squalene for targeting junction oncogenes in thyroid and іnvolvement of molecular chaperon (HSP 60 & 70) in thyroid cancer progression” (scientific supervisors – Professor Massaud Lilian, Institute Gustave-Roussy, Paris, France and Sidorik Lyudmila, IMBG NASU, Kyiv, Ukraine)

Collaboration:

with Ukrainian organizations:

  • National Scientific Center “M. D. Strazhesko Institute of Cardiology, NAMS of Ukraine” (Kyiv)
  • M. M. Amosov National Institute of Cardiovascular Surgery, NAMSU (Kyiv)
  • State Institution “Scientific-Practical Medical Center of Pediatric Cardiology and Cardiac Surgery” of the Health Ministry of Ukraine (Kyiv)
  • R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU (Kyiv)
  • O. V. Palladin Institute of Biochemistry, NASU (Kyiv)
  • Іnstitute of Cell Biology, NASU (Lviv)
  • Taras Shevchenko National University of Kyiv (Kyiv)
  • Morphological Laboratory “BIONTEC” (Dnepropetrovsk)
  • Bogomoletz Institute of Physiology, NASU (Kyiv)

with foreign organizations:

  • A. N. Bach Institute of Biochemistry, RAS (Moscow, Russia)
  • M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, RAS (Moscow, Russia)
  • Kirensky Institute of Physics of Siberian Branch, RAS (Krasnoyarsk, Russia)
  • International Institute of Molecular and Cell Biology (Warsaw, Poland)
  • Nencki Institute of Experimental Biology, PAS (Warsaw, Poland)
  • Institute Gustave-Roussy (Paris, France)

Selected publications:

  1. Makarenko M.V., Govsieiev D.O., Vorona R.М., Sydorik L.L., Yakovenko L.F., Krupskaya I.V., Pogribna А.P., Tsisarenko A.М., Pavlyuk O.V., Vasylchuk O.Ya. Identification methods and characteristics of antibodies for Hsp60 in pregnant women. International Journal Of Pharmacy & Technology. – 2016, 8 (2): 14666-14673.
  2. Grom MYu, Yakovenko LF, Granich VM, Dobrohod AS, Torbas OO, Radchenko GD, Sirenko YuM, Sidorik LL, Kornelyuk AI. Autoantibodies against tyrosyl-tRNA synthetase and its separated domains at essential hypertention. Bioрolym. & Cell. 2015;31(4):255-263.
  3. Kapustian LM, Kroupskaya IV, Rozhko OT, et al. The changes in Akt1 expression and activity at different stages of heart failure: experimental study. Pathophysiology. 2014;(21):147–151.
  4. Kapustian L, Kroupskaya I, Rozhko O, Bobyk V, Ryabenko D, Sidorik L. Akt1 expression and activity at different stages in experimental heart failure. Pathophysiology. 2013 doi:10.1016/j.pathophys.2013.11.005
  5. Kapustian LL, Vigontina OA, Rozhko OT et al. Hsp90 and its cochaperone, Sgt1, as autoantigens in dilated cardiomyopathy. Heart Vessels. 2013;28(1):114–9 doi:10.1007/s00380-011-0226-1
  6. Bobyk VI, Kapustian LN, Morozova LM, et al. Estimation of autoimmune and DNA-damage influence of gold nanoparticles. Biotechnology. 2012; 5(2):115–21.
  7. Rozhko OT, Kapustian LN, Bobyk VI, et al. Investigation of p70S6 kinase expression and localization at heart failure progression. Biopolym. Cell. 2010. 26(6):486–491 doi:10.7124/bc.000178
  8. Kondratyuk Y, Sidorik L, Bobyk V, Ryabenko D, Kornelyuk A. Autoantibodies against tyrosyl-tRNA synthetase at heart failure. Biopolym. Cell. 2010; 26(5):373–7 doi:10.7124/bc.00016B
  9. Kroupskaya IV, Kapustian LN, Sidorik LL. Bioinformatic prediction of phosphorylated sites of integrin b1-coupled protein melusin. Biopolym. Cell. 2008; 24(6):503–7 doi:10.7124/bc.0007C3
  10. Bobyk VI, Ryabenko DV, Sergienko OV, et al. Development of unique experimental model of autoimmune myosine-induced injury of myocardium. Biopolym. Cell. 2007; 23(2):115–21 doi:10.7124/bc.00075D
  11. Sidorik L, Kyyamova R, Bobyk V et al. Molecular chaperon HSP60 and cytochrome P450 2E1 co-expression in dilated cardiomyopathy. Cell Biology International. 2005; 29:51–5 doi:10.1016/j.cellbi.2004.11.011