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: (380-44) 200-03-37;
: (380-44) 526-07-59;
E-mail: negrutskii@imbg.org.ua

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  • 20122014 PICS (Projet International de Coopération Scientifique) Translation apparatus in cytosol: role in mitochondrial translation and antioxidant homeostasis
  • 20122013 5507 STCU (Science and Technology Center in Ukraine) The A2 isoform of translation elongation factor 1 as a potential oncomarker supported
  • 20112014 (7) FP7- INCO-2011-6, ERA-WIDE. : , COMBIOM ( ..)
  • 20102011 UKR10/A10 Structural and biological studies of the eukaryotic elongation factors (eEF) 1B, BMBF (Bundesministerium für Bildung und Forschung), ͳ
  • 20092012 GDRI. Earlier steps in the development of oncological, autoimmune and neurodegenerative diseases
  • 20092011 . Investigation of post-translational modifications of the components of human translation machinery during carcinogenesis
  • 20092011 PICS (Projet International de Coopération Scientifique). Structural and Functional organization of the translation apparatus in the cytoplasm of human cells
  • 20082009 . Structural and functional study of the two tissue-specific forms of eukaryotic elongation factor eEF1A
  • 20062008 Wellcome Trust. N 074742/Z04/Z Two-faced Janus: mammalian translation elongation factor 1A and its isoforms
  • 20062008 INTAS. 05-7750 Multisubunit translation elongation factor 1 in health and carcinogenesis
  • 20052007 . Thermodynamical analysis of the structure of the elongation factors domains in prokaryotes and eukaryotes

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  1. Bondarchuk TV, Lozhko DM, Shalak VF, Fatalska A, Szczepanowski RH, Dadlez M, Negrutskii BS, El'skaya AV. The protein-binding N-terminal domain of human translation elongation factor 1Bβ possesses a dynamic α-helical structural organization. / Int J Biol Macromol. 2019 Apr 1;126:899-907. doi: 10.1016/j.ijbiomac.2018.12.220. Epub 2018 Dec 24
  2. Bondarchuk T.V,, Lozhko D.M., Shalak V.F., Fatalska A., Szczepanowski R.H., Dadlez M., Negrutskii B.S., Elskaya A.V. The protein-binding N-terminal domain of human translation elongation factor 1Bβ possesses a dynamic α-helical structural organization. International Journal of Biological Macromolecules. 2018 Dec 24;126:899-907. doi: 10.1016/j.ijbiomac.2018.12.220
  3. Negrutskii B, Vlasenko D, Mirande M, Futernyk P, El'skaya A. mRNA-Independent way to regulate translation elongation rate in eukaryotic cells. IUBMB Life. 2018;70(3):192-196
  4. L. M. Kapustian, M. Dadlez, B. S. Negrutskii. Protein partners of the eEF1Bβ subunit of the translation elongation complex eEF1B in the nuclear fraction of human lung carcinoma cells. Biopolym. Cell. 2017;33(4):243-255
  5. Negrutskii B., Vlasenko D., Mirande M., Futernyk P., El'skaya A. mRNA-Independent way to regulate translation elongation rate in eukaryotic cells. International Union of Biochemistry and Molecular Biology Life. 2018 Mar;70(3):192-196
  6. Novosylna O, Doyle A, Vlasenko D, Murphy M, Negrutskii B, El'skaya A. Comparison of the ability of mammalian eEF1A1 and its oncogenic variant eEF1A2 to interact with actin and calmodulin. Biol. Chem. 2017;398(1):113-124
  7. Trosiuk TV, Shalak VF, Szczepanowski RH, Negrutskii BS, El'skaya AV. A non-catalytic N-terminal domain negatively influences the nucleotide exchange activity of translation elongation factor 1Bα. FEBS J. 2016 Feb; 283(3):484-97.
  8. Novosylna O, Jurewicz E, Pydiura N, Goral A, Filipek A, Negrutskii B, El'skaya A. Translation elongation factor eEF1A1 is a novel partner of a multifunctional protein Sgt1. Biochimie. 2015, Dec;119:137-45.
  9. Vlasenko DO, Novosylna OV, Negrutskii BS, El'skaya AV. Truncation of the A,A(∗),A' helices segment impairs the actin bundling activity of mammalian eEF1A1.FEBS Lett. 2015;589(11):1187-1193.
  10. Veremieva M, Kapustian L, Khoruzhenko A, Zakharychev V, Negrutskii B, El'skaya A. Independent overexpression of the subunits of translation elongation factor complex eEF1H in human lung cancer. BMC Cancer. 2014 Dec 3;14:913.
  11. Shalak V, Vislovukh A, Novosylna O, Khoruzhenko A...Negrutskii B. Characterization of novel peptide-specific antibodies against the translation elongation factor eEF1A2 and their use in eEF1A2 localization in cancer tissues. Biopolymers and Cell.2014;30(6):454-461.
  12. Trosiuk TV, Liudkovska VV, Shalak VF, Negrutskii1 BS, Elskaya AV. Structural dissection of human translation elongation factor 1Bγ (eEF1Bγ): expression of full-length protein and its truncated forms. Biopolymers and Cell. 2014;30(2):96-106.
  13. Thibaut Crepin, Shalak VF, Yaremchuk AD, ...El'skaya AV. Mammalian translation elongation factor eEF1A2: X-ray structure and new features of GDP/GTP exchange mechanism in higher eukaryotes. Nucleic Acids Research. 2014;42(20):12939-48.
  14. Vislovukh A, Thaiz Rivera Vargas, Polesskaya A, Groisman I. Role of 3-untranslated region translational control in cancer development, diagnostics and treatment. World Journal of Biological Chemistry. 2014;5:4057. doi:10.4331/wjbc.v5.i1.40
  15. El'skaya AV, Negrutskii BS, Shalak VF, et al. Specific features of protein biosynthesis in higher eukaryotes Biopolym. Cell. 2013; 29(3):177-187 doi: 10.7124/bc.000818
  16. Vislovukh A, Kratassiouk G, Porto E, Gralievska N, Beldiman C, Pinna G, El'skaya A, HarelBellan A, Negrutskii B, Groisman I. Protooncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR663 and miR744. Br J Cancer. 2013 Jun 11;108(11):230411. doi: 10.1038/bjc.2013.243
  17. Negrutskii B, Vlasenko D, El'skaya A. From global phosphoproteo mics to individual proteins: the case of translation elongation factor eEF1A. Expert Rev. Proteomics. 2012; 9(1):7183. doi: 10.1586/epr.11.71
  18. Yaremchuk A, Shalak VF, Novosylna OV, et al. Purification, crystallization and preliminary Xray crystallographic analysis of mammalian translation n elongation factor eEF1A2. Acta Cryst. 2012; F68:2957. doi: 10.1107/S1744309112000243
  19. Havrylenko S, Legouis R, Negrutskii B, Mirande M. Caenorhabditis elegans evolves a new architecture for the multiaminoacyl tRNA synthetase complex. J. Biol. Chem. 2011; 286:2847687. doi: 10.1074/jbc.M111.254037
  20. Veremieva M, Khoruzhenko A, Zaicev S, Negrutskii B, El'skaya A. Unbalanced expression of the translation complex eEF1 subunits in human cardioesophageal carcinoma. Eur. J. Clin. Invest. 2011; 41:26976. doi: 10.1111/j.1365-2362.2010.02404.x
  21. Kaminska M, Havrylenko S, Decottignies P, Le Marachal P, Negrutskii B, Mirande M. Dynamic organization of aminoacyl trna synthetase complexes in the cytoplasm of human cells. J. Biol. Chem. 2009; 284: 1374654. doi: 10.1074/jbc.M900480200
  22. Kaminska M, Havrylenko S, Decottignies P, et al. Dissection of the structural organization of the aminoacyltRNA synthetase complex. J. Biol. Chem. 2009. 284:605360. doi: 10.1074/jbc.M809636200
  23. Budkevich TV, El'skaya AV, Nierhaus KH. Features of 80S mammalian ribosome and its subunits. Nucleic Acids Res. 2009. 36:473644. doi: 10.1093/nar/gkn424
  24. Negrutskii BS, El'skaya AV. Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyltRNA channeling. Prog. Nucleic Acid Res. Mol. Biol. 1998; 60:4778. doi: 10.1016/S0079-6603(08)60889-2